A Simplified Screening Test for 4-Hydroxy-3-Methoxymandelic Acid (VMA) in Urine
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چکیده
منابع مشابه
Paper-chromatographic quantitation of 4-hydroxy-3-methoxymandelic acid (VMA) in urine.
We describe a quantitative paperchromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p-nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of rep...
متن کاملThe estimation of 4-hydroxy-3-methoxymandelic acid in urine.
Armstrong & McMillan (1957) identified 4hydroxy-3-methoxymandelic acid (vanilmandelic acid) in normal human urine and demonstrated that increased quantities were excreted after noradrenaline infusion and by patients with the catecholamine-secreting tumour, phaeochromocytoma. Subsequent work has established that 3methoxylation followed by oxidative deamination to 4-hydroxy-3-methoxymandelic acid...
متن کاملDEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY
We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...
متن کاملSimultaneous liquid-chromatographic analysis for 4-hydroxy-3-methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine.
We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0...
متن کاملdevelopment of a method for the determination of 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid) in urine by paper chromatography
we have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (vma) in urine. concentrations of vma in patients with neuroblastoma were increased in comparison to controls. the linearity was excellent in the concentration range tested. the within-assay coefficient of variation for control and patient urine was less than 2.2%. the r...
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ژورنال
عنوان ژورنال: Proceedings of the Association of Clinical Biochemists
سال: 1962
ISSN: 0369-8564
DOI: 10.1177/036985646200200221